DETAIL : GENE EXPRESSION

He established baculovirus insect cell expression system (BEVS) for the first time in India and used this to express foreign genes (>50) and also to ask fundamental questions concerning gene expression in BEVS.

• Demonstrated correct processing and intracellular deposition of expressed proteins;
• Discovered the phenomenon of secretory load;
• Developed BEVS as a system to study role of sugars in protein processing, folding and bioactivity;
• Discovered novel AUG context sequence which together with codon usage profile affected gene expression;
• Identified novel host factors which play critical role in regulating transcription from polyhedrin gene promoter;
• Discovered novel DNA enhancerelement, which upregulates gene expression >50 fold. (US Patented)